Is going alright.. there was a midterm on Tuesday, and it wasn’t too hard. There were some questions where I just had no idea though. Apparently, the question about which aligner used de Bruijn was a give away question, but I was very uncertain. Fortunately I guessed Velvet correctly!
There was a question about describing library construction and that question was awesome because I knew the answer too well…
New beads for bead clean up (DNA purification) were made recently. However, I’m surprised the beads we make aren’t contaminated because we use glassware and other stuff that to my knowledge isn’t exactly “DNA-free”.
Moreover, people are handling the microcentrifuge tubes without gloves before they go into the autoclave.. because they think autoclaving gets rid of DNA. However, it does not.
Anyway, we’re about to begin library construction on Monday on some important samples that cost a lot of moneys. We were supposed to start on Friday but other ppl in the lab weren’t ready.